AUTHORS :

MODELS : RHE / RECONSTRUCTED HUMAN EPIDERMIS

APPLICATIONS : Anti-Aging DNA Damage SKIN METABOLISM

2019 Elsevier - Toxicology Letters 305 (2019) 94-102
Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d’Opale, 59140, Dunkerque, France; Centre Commun de Mesures (CCM), Université du Littoral Côte d’Opale, 59140, Dunkerque, France; Laboratoires Clarins, 95300, Pontoise, France; INSERM UMR 1153 - CRESS, HERA (Health Environmental Risk Assessment) Research Unit, Paris Descartes-Sorbonne Paris Cité University, Paris, France; AP-HP Hôpital Bichat, Laboratoire de Biochimie, 75018, Paris, France

An in vitro model to evaluate the impact of environmental fine particles on skin damage

An in vitro model to evaluate the impact of environmental fine particles (PM0.3-2.5) on skin damage

A B S T R A C T

Exposure to airborne particulate matter (PM) has significant effects on human health mainly leading to cardiorespiratory diseases. However very few data are available regarding the impact of PM on the skin, so to better understand the impact of fine particle (PM0.3-2.5) on both inflammatory response and epidermal structure, we exposed a reconstructed human epidermis (RHE) to several doses of PM collected in Cotonou (Benin, West Africa). After 24 h of exposure, inflammatory response, histological observations, and gene expression related to oxidative stress, antioxidant defense and structural damages were determined. No PM-linked changes in tissue morphology or membrane integrity were observable. PM was however cytotoxic in a dose dependent manner. An inflammatory response appeared as shown by the increase in IL-1α and IL-8 cytokine productions. PM also induced oxidative stress, leading to an increase in 4-HNE immunostaining and to the up-regulation of HMOX1, MT1G and MT1E. Finally, PM had a negative impact on fundamental skin functions such as tissue anchorage, cell differentiation, cornification / skin desquamation and apoptosis. Our data show that airborne fine particles have an adverse effect on skin integrity, most probably leading to accelerated ageing.